Construct: shRNA TRCN0000232134

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003152.2-2479s21c1
DNA Barcode:
GGACCTTCTTGTTGCGCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAT5A (6776)
Gene Description:
signal transducer and activator of transcription 5A
Transcript:
RefSeq NM_003152.2 (NON-CURRENT)
Match location:
Position 2479 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232134)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACCTTCTTGTTGCGCTTTA
Hairpin Sequence:
5'-CCGG-GGACCTTCTTGTTGCGCTTTA-CTCGAG-TAAAGCGCAACAAGAAGGTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACCTTCTTGTTGCGCTTTACTCGAGTAAAGCGCAACAAGAAGGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACCTTCTTGTTGCGCTTTACTCGAGTAAAGCGCAACAAGAAGGTCC-3'

Other clones with same target sequence:

(none)