Construct: shRNA TRCN0000232139

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012448.3-486s21c1
DNA Barcode:
TCCGCTGCATCCGCCATATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAT5B (6777)
Gene Description:
signal transducer and activator of transcription 5B
Transcript:
RefSeq NM_012448.3 (NON-CURRENT)
Match location:
Position 486 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232139)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCGCTGCATCCGCCATATAT
Hairpin Sequence:
5'-CCGG-TCCGCTGCATCCGCCATATAT-CTCGAG-ATATATGGCGGATGCAGCGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCGCTGCATCCGCCATATATCTCGAGATATATGGCGGATGCAGCGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCGCTGCATCCGCCATATATCTCGAGATATATGGCGGATGCAGCGGA-3'

Other clones with same target sequence:

(none)