Construct: shRNA TRCN0000232169

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021916.2-1766s21c1
DNA Barcode:
TCGCCATCAGAAGATTCATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF70 (7621)
Gene Description:
zinc finger protein 70
Transcript:
RefSeq NM_021916.2 (NON-CURRENT)
Match location:
Position 1766 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232169)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGCCATCAGAAGATTCATTC
Hairpin Sequence:
5'-CCGG-TCGCCATCAGAAGATTCATTC-CTCGAG-GAATGAATCTTCTGATGGCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGCCATCAGAAGATTCATTCCTCGAGGAATGAATCTTCTGATGGCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGCCATCAGAAGATTCATTCCTCGAGGAATGAATCTTCTGATGGCGA-3'

Other clones with same target sequence:

(none)