Construct: shRNA TRCN0000232259

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019963.1-1325s21c1
DNA Barcode:
GGCCAGAGACAGGGCTTAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stat2 (20847)
Gene Description:
signal transducer and activator of transcription 2
Transcript:
RefSeq NM_019963.1 (CURRENT)
Match location:
Position 1325 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232259)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCCAGAGACAGGGCTTAATT
Hairpin Sequence:
5'-CCGG-GGCCAGAGACAGGGCTTAATT-CTCGAG-AATTAAGCCCTGTCTCTGGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCCAGAGACAGGGCTTAATTCTCGAGAATTAAGCCCTGTCTCTGGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCCAGAGACAGGGCTTAATTCTCGAGAATTAAGCCCTGTCTCTGGCC-3'

Other clones with same target sequence:

(none)