Construct: shRNA TRCN0000232292

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021045.1-950s21c1
DNA Barcode:
TCGGGCTTAATTATTAGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF248 (57209)
Gene Description:
zinc finger protein 248
Transcript:
RefSeq NM_021045.1 (NON-CURRENT)
Match location:
Position 950 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232292)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGGGCTTAATTATTAGTAAA
Hairpin Sequence:
5'-CCGG-TCGGGCTTAATTATTAGTAAA-CTCGAG-TTTACTAATAATTAAGCCCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGGGCTTAATTATTAGTAAACTCGAGTTTACTAATAATTAAGCCCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGGGCTTAATTATTAGTAAACTCGAGTTTACTAATAATTAAGCCCGA-3'

Other clones with same target sequence:

(none)