Construct: shRNA TRCN0000232381

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008551.1-398s21c1
DNA Barcode:
TCGATGGTGGAGAGCTCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mapkapk2 (17164)
Gene Description:
MAP kinase-activated protein kinase 2
Transcript:
RefSeq NM_008551.1 (NON-CURRENT)
Match location:
Position 398 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232381)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGATGGTGGAGAGCTCTTTA
Hairpin Sequence:
5'-CCGG-TCGATGGTGGAGAGCTCTTTA-CTCGAG-TAAAGAGCTCTCCACCATCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGATGGTGGAGAGCTCTTTACTCGAGTAAAGAGCTCTCCACCATCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGATGGTGGAGAGCTCTTTACTCGAGTAAAGAGCTCTCCACCATCGA-3'

Other clones with same target sequence:

(none)