Construct: shRNA TRCN0000232409

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021343.1-2098s21c1
DNA Barcode:
GTTGAACTTTCTAGCTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spata5 (57815)
Gene Description:
spermatogenesis associated 5
Transcript:
RefSeq NM_021343.1 (NON-CURRENT)
Match location:
Position 2098 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232409)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGAACTTTCTAGCTATAAA
Hairpin Sequence:
5'-CCGG-GTTGAACTTTCTAGCTATAAA-CTCGAG-TTTATAGCTAGAAAGTTCAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGAACTTTCTAGCTATAAACTCGAGTTTATAGCTAGAAAGTTCAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGAACTTTCTAGCTATAAACTCGAGTTTATAGCTAGAAAGTTCAAC-3'

Other clones with same target sequence:

(none)