Construct: shRNA TRCN0000232439

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022779.7-2799s21c1
DNA Barcode:
ACTGCGCCTTTGCCTGAATAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDX31 (64794)
Gene Description:
DEAD-box helicase 31
Transcript:
RefSeq NM_022779.7 (NON-CURRENT)
Match location:
Position 2799 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232439)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGCGCCTTTGCCTGAATAC
Hairpin Sequence:
5'-CCGG-ACTGCGCCTTTGCCTGAATAC-CTCGAG-GTATTCAGGCAAAGGCGCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGCGCCTTTGCCTGAATACCTCGAGGTATTCAGGCAAAGGCGCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGCGCCTTTGCCTGAATACCTCGAGGTATTCAGGCAAAGGCGCAGT-3'

Other clones with same target sequence:

(none)