Construct: shRNA TRCN0000232443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003543.3-131s21c1
DNA Barcode:
TCAAGCGAATTTCTGGCCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H4C8 (8365)
Gene Description:
H4 clustered histone 8
Transcript:
RefSeq NM_003543.3 (CURRENT)
Match location:
Position 131 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAAGCGAATTTCTGGCCTTA
Hairpin Sequence:
5'-CCGG-TCAAGCGAATTTCTGGCCTTA-CTCGAG-TAAGGCCAGAAATTCGCTTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAAGCGAATTTCTGGCCTTACTCGAGTAAGGCCAGAAATTCGCTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAAGCGAATTTCTGGCCTTACTCGAGTAAGGCCAGAAATTCGCTTGA-3'

Other clones with same target sequence:

(none)