Construct: shRNA TRCN0000232461

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021253.2-1955s21c1
DNA Barcode:
ACCCAAGCGGGTAGGCATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIM39 (56658)
Gene Description:
tripartite motif containing 39
Transcript:
RefSeq NM_021253.2 (NON-CURRENT)
Match location:
Position 1955 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232461)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCAAGCGGGTAGGCATATT
Hairpin Sequence:
5'-CCGG-ACCCAAGCGGGTAGGCATATT-CTCGAG-AATATGCCTACCCGCTTGGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCAAGCGGGTAGGCATATTCTCGAGAATATGCCTACCCGCTTGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCAAGCGGGTAGGCATATTCTCGAGAATATGCCTACCCGCTTGGGT-3'

Other clones with same target sequence:

(none)