Construct: shRNA TRCN0000232572

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020850.1-974s21c1
DNA Barcode:
GGAAGAACAGGCGTCCATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RANBP10 (57610)
Gene Description:
RAN binding protein 10
Transcript:
RefSeq NM_020850.1 (NON-CURRENT)
Match location:
Position 974 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232572)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAAGAACAGGCGTCCATAAA
Hairpin Sequence:
5'-CCGG-GGAAGAACAGGCGTCCATAAA-CTCGAG-TTTATGGACGCCTGTTCTTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAAGAACAGGCGTCCATAAACTCGAGTTTATGGACGCCTGTTCTTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAAGAACAGGCGTCCATAAACTCGAGTTTATGGACGCCTGTTCTTCC-3'

Other clones with same target sequence:

(none)