Construct: shRNA TRCN0000232609

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024678.3-1817s21c1
DNA Barcode:
CCCGCAAGAAGAGGTACTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NARS2 (79731)
Gene Description:
asparaginyl-tRNA synthetase 2, mitochondrial
Transcript:
RefSeq NM_024678.3 (NON-CURRENT)
Match location:
Position 1817 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232609)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGCAAGAAGAGGTACTTAT
Hairpin Sequence:
5'-CCGG-CCCGCAAGAAGAGGTACTTAT-CTCGAG-ATAAGTACCTCTTCTTGCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGCAAGAAGAGGTACTTATCTCGAGATAAGTACCTCTTCTTGCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGCAAGAAGAGGTACTTATCTCGAGATAAGTACCTCTTCTTGCGGG-3'

Other clones with same target sequence:

  1. TRCN0000163609