Construct: shRNA TRCN0000232677

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031283.1-511s21c1
DNA Barcode:
GCACCTACCTGCAGATGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TCF7L1 (83439)
Gene Description:
transcription factor 7 like 1
Transcript:
RefSeq NM_031283.1 (NON-CURRENT)
Match location:
Position 511 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232677)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCTACCTGCAGATGAAAT
Hairpin Sequence:
5'-CCGG-GCACCTACCTGCAGATGAAAT-CTCGAG-ATTTCATCTGCAGGTAGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCTACCTGCAGATGAAATCTCGAGATTTCATCTGCAGGTAGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCTACCTGCAGATGAAATCTCGAGATTTCATCTGCAGGTAGGTGC-3'

Other clones with same target sequence:

(none)