Construct: shRNA TRCN0000232711

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003689.2-1207s21c1
DNA Barcode:
GTCTAGATCCATGCATTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKR7A2 (8574)
Gene Description:
aldo-keto reductase family 7 member A2
Transcript:
RefSeq NM_003689.2 (NON-CURRENT)
Match location:
Position 1207 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232711)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTAGATCCATGCATTATTT
Hairpin Sequence:
5'-CCGG-GTCTAGATCCATGCATTATTT-CTCGAG-AAATAATGCATGGATCTAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTAGATCCATGCATTATTTCTCGAGAAATAATGCATGGATCTAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTAGATCCATGCATTATTTCTCGAGAAATAATGCATGGATCTAGAC-3'

Other clones with same target sequence:

(none)