Construct: shRNA TRCN0000232743

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018216.1-448s21c1
DNA Barcode:
GTGATGACGTGCCTGATTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PANK4 (55229)
Gene Description:
pantothenate kinase 4 (inactive)
Transcript:
RefSeq NM_018216.1 (NON-CURRENT)
Match location:
Position 448 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232743)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGATGACGTGCCTGATTAAG
Hairpin Sequence:
5'-CCGG-GTGATGACGTGCCTGATTAAG-CTCGAG-CTTAATCAGGCACGTCATCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGATGACGTGCCTGATTAAGCTCGAGCTTAATCAGGCACGTCATCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGATGACGTGCCTGATTAAGCTCGAGCTTAATCAGGCACGTCATCAC-3'

Other clones with same target sequence:

(none)