Construct: shRNA TRCN0000232966
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_005155.5-1035s21c1
- DNA Barcode:
- CCATGCGGTCTAACCTCTATC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PPT2 (9374)
- Gene Description:
- palmitoyl-protein thioesterase 2
- Transcript:
- RefSeq NM_005155.5 (NON-CURRENT)
- Match location:
- Position 1035 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000232966)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCATGCGGTCTAACCTCTATC
- Hairpin Sequence:
-
5'-CCGG-CCATGCGGTCTAACCTCTATC-CTCGAG-GATAGAGGTTAGACCGCATGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCATGCGGTCTAACCTCTATCCTCGAGGATAGAGGTTAGACCGCATGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCATGCGGTCTAACCTCTATCCTCGAGGATAGAGGTTAGACCGCATGG-3'
Other clones with same target sequence:
-
TRCN0000251106
