Construct: shRNA TRCN0000233056

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005113.2-2609s21c1
DNA Barcode:
GAGTTTAGTGGTCCTAATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GOLGA5 (9950)
Gene Description:
golgin A5
Transcript:
RefSeq NM_005113.2 (NON-CURRENT)
Match location:
Position 2609 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233056)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTTAGTGGTCCTAATATA
Hairpin Sequence:
5'-CCGG-GAGTTTAGTGGTCCTAATATA-CTCGAG-TATATTAGGACCACTAAACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTTAGTGGTCCTAATATACTCGAGTATATTAGGACCACTAAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTTAGTGGTCCTAATATACTCGAGTATATTAGGACCACTAAACTC-3'

Other clones with same target sequence:

(none)