Construct: shRNA TRCN0000233072

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006560.2-481s21c1
DNA Barcode:
AGCTGTTTATTGGTATGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CELF1 (10658)
Gene Description:
CUGBP Elav-like family member 1
Transcript:
RefSeq NM_006560.2 (NON-CURRENT)
Match location:
Position 481 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233072)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTGTTTATTGGTATGATTT
Hairpin Sequence:
5'-CCGG-AGCTGTTTATTGGTATGATTT-CTCGAG-AAATCATACCAATAAACAGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTGTTTATTGGTATGATTTCTCGAGAAATCATACCAATAAACAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTGTTTATTGGTATGATTTCTCGAGAAATCATACCAATAAACAGCT-3'

Other clones with same target sequence:

(none)