Construct: shRNA TRCN0000233076

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006560.2-1690s21c1
DNA Barcode:
CGTCAAGTACATCGTCCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CELF1 (10658)
Gene Description:
CUGBP Elav-like family member 1
Transcript:
RefSeq NM_006560.2 (NON-CURRENT)
Match location:
Position 1690 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233076)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCAAGTACATCGTCCAAAT
Hairpin Sequence:
5'-CCGG-CGTCAAGTACATCGTCCAAAT-CTCGAG-ATTTGGACGATGTACTTGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCAAGTACATCGTCCAAATCTCGAGATTTGGACGATGTACTTGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCAAGTACATCGTCCAAATCTCGAGATTTGGACGATGTACTTGACG-3'

Other clones with same target sequence:

  1. TRCN0000098510
  2. TRCN0000326898