Construct: shRNA TRCN0000233083

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021235.1-140s21c1
DNA Barcode:
AGTCTGGCCTCTCGGACATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPS15L1 (58513)
Gene Description:
epidermal growth factor receptor pathway substrate 15 like 1
Transcript:
RefSeq NM_021235.1 (NON-CURRENT)
Match location:
Position 140 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233083)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCTGGCCTCTCGGACATTA
Hairpin Sequence:
5'-CCGG-AGTCTGGCCTCTCGGACATTA-CTCGAG-TAATGTCCGAGAGGCCAGACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCTGGCCTCTCGGACATTACTCGAGTAATGTCCGAGAGGCCAGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCTGGCCTCTCGGACATTACTCGAGTAATGTCCGAGAGGCCAGACT-3'

Other clones with same target sequence:

(none)