Construct: shRNA TRCN0000233087

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014632.2-473s21c1
DNA Barcode:
CCAAAGCCCTGTGGTACAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MICAL2 (9645)
Gene Description:
microtubule associated monooxygenase, calponin and LIM domain containing 2
Transcript:
RefSeq NM_014632.2 (NON-CURRENT)
Match location:
Position 473 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233087)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGCCCTGTGGTACAAAT
Hairpin Sequence:
5'-CCGG-CCAAAGCCCTGTGGTACAAAT-CTCGAG-ATTTGTACCACAGGGCTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGCCCTGTGGTACAAATCTCGAGATTTGTACCACAGGGCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGCCCTGTGGTACAAATCTCGAGATTTGTACCACAGGGCTTTGG-3'

Other clones with same target sequence:

(none)