Construct: shRNA TRCN0000233107

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024537.1-358s21c1
DNA Barcode:
TAGTCATGGTGATGGGTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CARS2 (79587)
Gene Description:
cysteinyl-tRNA synthetase 2, mitochondrial
Transcript:
RefSeq NM_024537.1 (NON-CURRENT)
Match location:
Position 358 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233107)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGTCATGGTGATGGGTATTA
Hairpin Sequence:
5'-CCGG-TAGTCATGGTGATGGGTATTA-CTCGAG-TAATACCCATCACCATGACTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGTCATGGTGATGGGTATTACTCGAGTAATACCCATCACCATGACTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGTCATGGTGATGGGTATTACTCGAGTAATACCCATCACCATGACTA-3'

Other clones with same target sequence:

(none)