Construct: shRNA TRCN0000233163

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182314.1-1989s21c1
DNA Barcode:
TGAACAGCAGTCCTATCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ENOX2 (10495)
Gene Description:
ecto-NOX disulfide-thiol exchanger 2
Transcript:
RefSeq NM_182314.1 (NON-CURRENT)
Match location:
Position 1989 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233163)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAACAGCAGTCCTATCAAAT
Hairpin Sequence:
5'-CCGG-TGAACAGCAGTCCTATCAAAT-CTCGAG-ATTTGATAGGACTGCTGTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAACAGCAGTCCTATCAAATCTCGAGATTTGATAGGACTGCTGTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAACAGCAGTCCTATCAAATCTCGAGATTTGATAGGACTGCTGTTCA-3'

Other clones with same target sequence:

(none)