Construct: shRNA TRCN0000233184

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002692.2-1258s21c1
DNA Barcode:
TCCGTGAAGACTTAGTAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POLE2 (5427)
Gene Description:
DNA polymerase epsilon 2, accessory subunit
Transcript:
RefSeq NM_002692.2 (NON-CURRENT)
Match location:
Position 1258 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233184)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCGTGAAGACTTAGTAAATA
Hairpin Sequence:
5'-CCGG-TCCGTGAAGACTTAGTAAATA-CTCGAG-TATTTACTAAGTCTTCACGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCGTGAAGACTTAGTAAATACTCGAGTATTTACTAAGTCTTCACGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCGTGAAGACTTAGTAAATACTCGAGTATTTACTAAGTCTTCACGGA-3'

Other clones with same target sequence:

(none)