Construct: shRNA TRCN0000233237

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138418.2-724s21c1
DNA Barcode:
CACCTGAAGTGCCAGCATTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MCRIP2 (84331)
Gene Description:
MAPK regulated corepressor interacting protein 2
Transcript:
RefSeq NM_138418.2 (NON-CURRENT)
Match location:
Position 724 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233237)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCTGAAGTGCCAGCATTTG
Hairpin Sequence:
5'-CCGG-CACCTGAAGTGCCAGCATTTG-CTCGAG-CAAATGCTGGCACTTCAGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCTGAAGTGCCAGCATTTGCTCGAGCAAATGCTGGCACTTCAGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCTGAAGTGCCAGCATTTGCTCGAGCAAATGCTGGCACTTCAGGTG-3'

Other clones with same target sequence:

(none)