Construct: shRNA TRCN0000233273

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_170697.1-303s21c1
DNA Barcode:
TCCTGTAGATGGAGACTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALDH1A2 (8854)
Gene Description:
aldehyde dehydrogenase 1 family member A2
Transcript:
RefSeq NM_170697.1 (NON-CURRENT)
Match location:
Position 303 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233273)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTGTAGATGGAGACTATTT
Hairpin Sequence:
5'-CCGG-TCCTGTAGATGGAGACTATTT-CTCGAG-AAATAGTCTCCATCTACAGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTGTAGATGGAGACTATTTCTCGAGAAATAGTCTCCATCTACAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTGTAGATGGAGACTATTTCTCGAGAAATAGTCTCCATCTACAGGA-3'

Other clones with same target sequence:

(none)