Construct: shRNA TRCN0000233296
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001068.2-2554s21c1
- DNA Barcode:
- GTAGAGCCTGAGTGGTATATT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TOP2B (7155)
- Gene Description:
- DNA topoisomerase II beta
- Transcript:
- RefSeq NM_001068.2 (NON-CURRENT)
- Match location:
- Position 2554 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000233296)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTAGAGCCTGAGTGGTATATT
- Hairpin Sequence:
-
5'-CCGG-GTAGAGCCTGAGTGGTATATT-CTCGAG-AATATACCACTCAGGCTCTAC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTAGAGCCTGAGTGGTATATTCTCGAGAATATACCACTCAGGCTCTACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTAGAGCCTGAGTGGTATATTCTCGAGAATATACCACTCAGGCTCTAC-3'
Other clones with same target sequence:
(none)
