Construct: shRNA TRCN0000233321

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011512.2-1778s21c1
DNA Barcode:
CCTTAGCTCAAACTACTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Surf4 (20932)
Gene Description:
surfeit gene 4
Transcript:
RefSeq NM_011512.2 (NON-CURRENT)
Match location:
Position 1778 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233321)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTAGCTCAAACTACTAAAT
Hairpin Sequence:
5'-CCGG-CCTTAGCTCAAACTACTAAAT-CTCGAG-ATTTAGTAGTTTGAGCTAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTAGCTCAAACTACTAAATCTCGAGATTTAGTAGTTTGAGCTAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTAGCTCAAACTACTAAATCTCGAGATTTAGTAGTTTGAGCTAAGG-3'

Other clones with same target sequence:

  1. TRCN0000119382