Construct: shRNA TRCN0000233341

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175606.2-426s21c1
DNA Barcode:
CAGACGCAGAAATGGTTTAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hopx (74318)
Gene Description:
HOP homeobox
Transcript:
RefSeq NM_175606.2 (NON-CURRENT)
Match location:
Position 426 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233341)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGACGCAGAAATGGTTTAAG
Hairpin Sequence:
5'-CCGG-CAGACGCAGAAATGGTTTAAG-CTCGAG-CTTAAACCATTTCTGCGTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGACGCAGAAATGGTTTAAGCTCGAGCTTAAACCATTTCTGCGTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGACGCAGAAATGGTTTAAGCTCGAGCTTAAACCATTTCTGCGTCTG-3'

Other clones with same target sequence:

(none)