Construct: shRNA TRCN0000233416

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011548.3-2629s21c1
DNA Barcode:
GCACATCGTGCCTAAGCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tcf3 (21423)
Gene Description:
transcription factor 3
Transcript:
RefSeq NM_011548.3 (NON-CURRENT)
Match location:
Position 2629 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233416)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATCGTGCCTAAGCATTT
Hairpin Sequence:
5'-CCGG-GCACATCGTGCCTAAGCATTT-CTCGAG-AAATGCTTAGGCACGATGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATCGTGCCTAAGCATTTCTCGAGAAATGCTTAGGCACGATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATCGTGCCTAAGCATTTCTCGAGAAATGCTTAGGCACGATGTGC-3'

Other clones with same target sequence:

(none)