Construct: shRNA TRCN0000233539

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006532.2-1624s21c1
DNA Barcode:
GCTACAAGAACGACTTCAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ELL (8178)
Gene Description:
elongation factor for RNA polymerase II
Transcript:
RefSeq NM_006532.2 (NON-CURRENT)
Match location:
Position 1624 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233539)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACAAGAACGACTTCAATG
Hairpin Sequence:
5'-CCGG-GCTACAAGAACGACTTCAATG-CTCGAG-CATTGAAGTCGTTCTTGTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACAAGAACGACTTCAATGCTCGAGCATTGAAGTCGTTCTTGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACAAGAACGACTTCAATGCTCGAGCATTGAAGTCGTTCTTGTAGC-3'

Other clones with same target sequence:

(none)