Construct: shRNA TRCN0000233962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031877.2-187s21c1
DNA Barcode:
TGAGTAGCCTAAGTAAGTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wasf1 (83767)
Gene Description:
WAS protein family, member 1
Transcript:
RefSeq NM_031877.2 (NON-CURRENT)
Match location:
Position 187 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGTAGCCTAAGTAAGTATG
Hairpin Sequence:
5'-CCGG-TGAGTAGCCTAAGTAAGTATG-CTCGAG-CATACTTACTTAGGCTACTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGTAGCCTAAGTAAGTATGCTCGAGCATACTTACTTAGGCTACTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGTAGCCTAAGTAAGTATGCTCGAGCATACTTACTTAGGCTACTCA-3'

Other clones with same target sequence:

(none)