Construct: shRNA TRCN0000233978

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020614.1-1308s21c1
DNA Barcode:
CGATAAGTCCGTCGCATATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Taf1b (21340)
Gene Description:
TATA-box binding protein associated factor, RNA polymerase I, B
Transcript:
RefSeq NM_020614.1 (NON-CURRENT)
Match location:
Position 1308 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233978)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATAAGTCCGTCGCATATAA
Hairpin Sequence:
5'-CCGG-CGATAAGTCCGTCGCATATAA-CTCGAG-TTATATGCGACGGACTTATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATAAGTCCGTCGCATATAACTCGAGTTATATGCGACGGACTTATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATAAGTCCGTCGCATATAACTCGAGTTATATGCGACGGACTTATCG-3'

Other clones with same target sequence:

(none)