Construct: shRNA TRCN0000233988

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020618.3-740s21c1
DNA Barcode:
GGATACAATCCATACAGTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smarce1 (57376)
Gene Description:
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Transcript:
RefSeq NM_020618.3 (NON-CURRENT)
Match location:
Position 740 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233988)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATACAATCCATACAGTCAT
Hairpin Sequence:
5'-CCGG-GGATACAATCCATACAGTCAT-CTCGAG-ATGACTGTATGGATTGTATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATACAATCCATACAGTCATCTCGAGATGACTGTATGGATTGTATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATACAATCCATACAGTCATCTCGAGATGACTGTATGGATTGTATCC-3'

Other clones with same target sequence:

(none)