Construct: shRNA TRCN0000233990

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031183.1-326s21c1
DNA Barcode:
TAACCTGCGAGGACCTGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sp6 (83395)
Gene Description:
trans-acting transcription factor 6
Transcript:
RefSeq NM_031183.1 (NON-CURRENT)
Match location:
Position 326 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000233990)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAACCTGCGAGGACCTGGAAA
Hairpin Sequence:
5'-CCGG-TAACCTGCGAGGACCTGGAAA-CTCGAG-TTTCCAGGTCCTCGCAGGTTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAACCTGCGAGGACCTGGAAACTCGAGTTTCCAGGTCCTCGCAGGTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAACCTGCGAGGACCTGGAAACTCGAGTTTCCAGGTCCTCGCAGGTTA-3'

Other clones with same target sequence:

(none)