Construct: shRNA TRCN0000234016

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008904.1-1601s21c1
DNA Barcode:
CTAGCCATGGATGGCCTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppargc1a (19017)
Gene Description:
peroxisome proliferative activated receptor, gamma, coactivator 1 alpha
Transcript:
RefSeq NM_008904.1 (NON-CURRENT)
Match location:
Position 1601 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234016)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGCCATGGATGGCCTATTT
Hairpin Sequence:
5'-CCGG-CTAGCCATGGATGGCCTATTT-CTCGAG-AAATAGGCCATCCATGGCTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGCCATGGATGGCCTATTTCTCGAGAAATAGGCCATCCATGGCTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGCCATGGATGGCCTATTTCTCGAGAAATAGGCCATCCATGGCTAG-3'

Other clones with same target sequence:

(none)