Construct: shRNA TRCN0000234050

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022988.2-1007s21c1
DNA Barcode:
CTGCTTCCAAAGGGATCAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nif3l1 (65102)
Gene Description:
Ngg1 interacting factor 3-like 1 (S. pombe)
Transcript:
RefSeq NM_022988.2 (NON-CURRENT)
Match location:
Position 1007 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234050)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCTTCCAAAGGGATCAATG
Hairpin Sequence:
5'-CCGG-CTGCTTCCAAAGGGATCAATG-CTCGAG-CATTGATCCCTTTGGAAGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCTTCCAAAGGGATCAATGCTCGAGCATTGATCCCTTTGGAAGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCTTCCAAAGGGATCAATGCTCGAGCATTGATCCCTTTGGAAGCAG-3'

Other clones with same target sequence:

(none)