Construct: shRNA TRCN0000234050
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_022988.2-1007s21c1
- DNA Barcode:
- CTGCTTCCAAAGGGATCAATG
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Nif3l1 (65102)
- Gene Description:
- Ngg1 interacting factor 3-like 1 (S. pombe)
- Transcript:
- RefSeq NM_022988.2 (NON-CURRENT)
- Match location:
- Position 1007 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000234050)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTGCTTCCAAAGGGATCAATG
- Hairpin Sequence:
-
5'-CCGG-CTGCTTCCAAAGGGATCAATG-CTCGAG-CATTGATCCCTTTGGAAGCAG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTGCTTCCAAAGGGATCAATGCTCGAGCATTGATCCCTTTGGAAGCAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTGCTTCCAAAGGGATCAATGCTCGAGCATTGATCCCTTTGGAAGCAG-3'
Other clones with same target sequence:
(none)
