Construct: shRNA TRCN0000234073

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010484.1-1993s21c1
DNA Barcode:
ACACTTAAGGAGCGCATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc6a4 (15567)
Gene Description:
solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
Transcript:
RefSeq NM_010484.1 (NON-CURRENT)
Match location:
Position 1993 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234073)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACTTAAGGAGCGCATTATT
Hairpin Sequence:
5'-CCGG-ACACTTAAGGAGCGCATTATT-CTCGAG-AATAATGCGCTCCTTAAGTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACTTAAGGAGCGCATTATTCTCGAGAATAATGCGCTCCTTAAGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACTTAAGGAGCGCATTATTCTCGAGAATAATGCGCTCCTTAAGTGT-3'

Other clones with same target sequence:

(none)