Construct: shRNA TRCN0000234079

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011151.1-2021s21c1
DNA Barcode:
AGTTTCATGTGAGCAATTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppm1b (19043)
Gene Description:
protein phosphatase 1B, magnesium dependent, beta isoform
Transcript:
RefSeq NM_011151.1 (NON-CURRENT)
Match location:
Position 2021 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234079)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTTCATGTGAGCAATTTAC
Hairpin Sequence:
5'-CCGG-AGTTTCATGTGAGCAATTTAC-CTCGAG-GTAAATTGCTCACATGAAACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTTCATGTGAGCAATTTACCTCGAGGTAAATTGCTCACATGAAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTTCATGTGAGCAATTTACCTCGAGGTAAATTGCTCACATGAAACT-3'

Other clones with same target sequence:

(none)