Construct: shRNA TRCN0000234094

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199064.1-590s21c1
DNA Barcode:
TGGCATTCAGGTCAATGTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ssxb10 (385312)
Gene Description:
synovial sarcoma, X member B10
Transcript:
RefSeq NM_199064.1 (CURRENT)
Match location:
Position 590 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234094)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGCATTCAGGTCAATGTTTG
Hairpin Sequence:
5'-CCGG-TGGCATTCAGGTCAATGTTTG-CTCGAG-CAAACATTGACCTGAATGCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGCATTCAGGTCAATGTTTGCTCGAGCAAACATTGACCTGAATGCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGCATTCAGGTCAATGTTTGCTCGAGCAAACATTGACCTGAATGCCA-3'

Other clones with same target sequence:

(none)