Construct: shRNA TRCN0000234147

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_886491.1-77s21c1
DNA Barcode:
CAAAGTGTGTAAAGGACAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
EG546908 (546908)
Gene Description:
predicted gene, EG546908
Transcript:
RefSeq XM_886491.1 (NON-CURRENT)
Match location:
Position 77 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234147)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGTGTGTAAAGGACAAAC
Hairpin Sequence:
5'-CCGG-CAAAGTGTGTAAAGGACAAAC-CTCGAG-GTTTGTCCTTTACACACTTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGTGTGTAAAGGACAAACCTCGAGGTTTGTCCTTTACACACTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGTGTGTAAAGGACAAACCTCGAGGTTTGTCCTTTACACACTTTG-3'

Other clones with same target sequence:

(none)