Construct: shRNA TRCN0000234154

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_887994.1-790s21c1
DNA Barcode:
GAGGACGTCTTTGCATATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Med12l (329650)
Gene Description:
mediator complex subunit 12-like
Transcript:
RefSeq XM_887994.1 (NON-CURRENT)
Match location:
Position 790 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234154)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGACGTCTTTGCATATTTA
Hairpin Sequence:
5'-CCGG-GAGGACGTCTTTGCATATTTA-CTCGAG-TAAATATGCAAAGACGTCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGACGTCTTTGCATATTTACTCGAGTAAATATGCAAAGACGTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGACGTCTTTGCATATTTACTCGAGTAAATATGCAAAGACGTCCTC-3'

Other clones with same target sequence:

(none)