Construct: shRNA TRCN0000234257

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_987077.1-748s21c1
DNA Barcode:
ACAATGCTGAAAGATCTAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prune2 (77754)
Gene Description:
prune homolog 2 (Drosophila)
Transcript:
RefSeq XM_987077.1 (NON-CURRENT)
Match location:
Position 748 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234257)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAATGCTGAAAGATCTAAAG
Hairpin Sequence:
5'-CCGG-ACAATGCTGAAAGATCTAAAG-CTCGAG-CTTTAGATCTTTCAGCATTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAATGCTGAAAGATCTAAAGCTCGAGCTTTAGATCTTTCAGCATTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAATGCTGAAAGATCTAAAGCTCGAGCTTTAGATCTTTCAGCATTGT-3'

Other clones with same target sequence:

(none)