Construct: shRNA TRCN0000234261
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_001001824.1-946s21c1
- DNA Barcode:
- ATCCATTGTACTACAAGTTAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Gm12372 (545600)
- Gene Description:
- predicted gene 12372
- Transcript:
- RefSeq XM_001001824.1 (NON-CURRENT)
- Match location:
- Position 946 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000234261)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- ATCCATTGTACTACAAGTTAA
- Hairpin Sequence:
-
5'-CCGG-ATCCATTGTACTACAAGTTAA-CTCGAG-TTAACTTGTAGTACAATGGAT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGATCCATTGTACTACAAGTTAACTCGAGTTAACTTGTAGTACAATGGATTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAATCCATTGTACTACAAGTTAACTCGAGTTAACTTGTAGTACAATGGAT-3'
Other clones with same target sequence:
(none)
