Construct: shRNA TRCN0000234301

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001003759.1-267s21c1
DNA Barcode:
CCTTCTCTGTCACATCATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm9717 (677457)
Gene Description:
predicted gene 9717; MAS-related GPR, member A4 pseudogene
Transcript:
RefSeq XM_001003759.1 (NON-CURRENT)
Match location:
Position 267 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234301)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCTCTGTCACATCATAAA
Hairpin Sequence:
5'-CCGG-CCTTCTCTGTCACATCATAAA-CTCGAG-TTTATGATGTGACAGAGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCTCTGTCACATCATAAACTCGAGTTTATGATGTGACAGAGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCTCTGTCACATCATAAACTCGAGTTTATGATGTGACAGAGAAGG-3'

Other clones with same target sequence:

(none)