Construct: shRNA TRCN0000234310

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023934.3-2508s21c1
DNA Barcode:
TGCAAGGTTCACATCGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FUNDC2 (65991)
Gene Description:
FUN14 domain containing 2
Transcript:
RefSeq NM_023934.3 (NON-CURRENT)
Match location:
Position 2508 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234310)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCAAGGTTCACATCGTATTT
Hairpin Sequence:
5'-CCGG-TGCAAGGTTCACATCGTATTT-CTCGAG-AAATACGATGTGAACCTTGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCAAGGTTCACATCGTATTTCTCGAGAAATACGATGTGAACCTTGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCAAGGTTCACATCGTATTTCTCGAGAAATACGATGTGAACCTTGCA-3'

Other clones with same target sequence:

(none)