Construct: shRNA TRCN0000234347

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003410.2-953s21c1
DNA Barcode:
TGGACACAGAGTCGGAAATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZFX (7543)
Gene Description:
zinc finger protein X-linked
Transcript:
RefSeq NM_003410.2 (NON-CURRENT)
Match location:
Position 953 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234347)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGACACAGAGTCGGAAATTG
Hairpin Sequence:
5'-CCGG-TGGACACAGAGTCGGAAATTG-CTCGAG-CAATTTCCGACTCTGTGTCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGACACAGAGTCGGAAATTGCTCGAGCAATTTCCGACTCTGTGTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGACACAGAGTCGGAAATTGCTCGAGCAATTTCCGACTCTGTGTCCA-3'

Other clones with same target sequence:

(none)