Construct: shRNA TRCN0000234386

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016733.2-1607s21c1
DNA Barcode:
ATGGCCTATTTGCACTCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIMK2 (3985)
Gene Description:
LIM domain kinase 2
Transcript:
RefSeq NM_016733.2 (NON-CURRENT)
Match location:
Position 1607 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234386)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGCCTATTTGCACTCTATG
Hairpin Sequence:
5'-CCGG-ATGGCCTATTTGCACTCTATG-CTCGAG-CATAGAGTGCAAATAGGCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGCCTATTTGCACTCTATGCTCGAGCATAGAGTGCAAATAGGCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGCCTATTTGCACTCTATGCTCGAGCATAGAGTGCAAATAGGCCAT-3'

Other clones with same target sequence:

(none)