Construct: shRNA TRCN0000234425

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011817.1-307s21c1
DNA Barcode:
ATAGCGCTGCAGATCCATTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gadd45g (23882)
Gene Description:
growth arrest and DNA-damage-inducible 45 gamma
Transcript:
RefSeq NM_011817.1 (NON-CURRENT)
Match location:
Position 307 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234425)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATAGCGCTGCAGATCCATTTC
Hairpin Sequence:
5'-CCGG-ATAGCGCTGCAGATCCATTTC-CTCGAG-GAAATGGATCTGCAGCGCTAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATAGCGCTGCAGATCCATTTCCTCGAGGAAATGGATCTGCAGCGCTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATAGCGCTGCAGATCCATTTCCTCGAGGAAATGGATCTGCAGCGCTAT-3'

Other clones with same target sequence:

(none)