Construct: shRNA TRCN0000234429

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029976.2-238s21c1
DNA Barcode:
TGGACCAGCTGCTGTCCTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdkn2aipnl (52626)
Gene Description:
CDKN2A interacting protein N-terminal like
Transcript:
RefSeq NM_029976.2 (NON-CURRENT)
Match location:
Position 238 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234429)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGACCAGCTGCTGTCCTTAT
Hairpin Sequence:
5'-CCGG-TGGACCAGCTGCTGTCCTTAT-CTCGAG-ATAAGGACAGCAGCTGGTCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGACCAGCTGCTGTCCTTATCTCGAGATAAGGACAGCAGCTGGTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGACCAGCTGCTGTCCTTATCTCGAGATAAGGACAGCAGCTGGTCCA-3'

Other clones with same target sequence:

(none)